GENETIC TECHNOLOGY REVIEW FOR “QUEST”

Word Bank: words may be used more than once or not at all

Plasmids Restriction Enzymes DNA

Nitrogen Base Sequences Point Mutation Vector

Recombinant DNA Gel Electrophoresis

Pulled by the Agarose Gel Attracted to the Oppositely Charged Pole

Separated by Size DNA Fingerprint Band

Smallest Fragments Largest Fragments Bacteria Cells

The Human Immune System Sticky Ends

Transgenic Organism Amino Acid Sequences Jagged Ends

Complete the following statements by filling in the blank with the appropriate choice from the Bank.

1. PLASMIDS are circular pieces of DNA found in BACTERIA CELLS.

2. GEL ELECTROPHORESIS separates fragments of DNA that have been cut by RESTRICTION ENZYMES.

3. RESTRICTION ENZYMES cut DNA at specific NITROGEN BASE SEQUENCES.

4. RECOMBINANT DNA contains DNA from more than one source.

5. A POINT MUTATION in a DNA sequence may affect the action of RESTRICTION ENZYMES. The required sequence for cleaving may no longer be present in the DNA.

6. In GEL ELECTROPHORESIS, the fragments of DNA are separated because they are ATTRACTED TO THE OPPOSITELY CHARGED POLE.

7. Each BAND on a gel represents one fragment of DNA.

8. A VECTOR is the means by which recombinant DNA can be transferred into an organism. Usually, a bacterial PLASMID is used.

9. In order to recombine, each segment of DNA must have STICKY ENDS.

10. The pattern produced in a DNA FINGERPRINT results from the DNA being SEPARATED BY SIZE.

11. In gel electrophoresis, the SMALLEST FRAGMENTS move farthest from the well.

12. The reason gel electrophoresis can produce a DNA FINGERPRINT is because the fragments of DNA are SEPARATED BY SIZE.

13. When DNA from more than one source, RECOMBINANT DNA, is placed inside an organism, that organism can now be called a TRANSGENIC ORGANISM.